| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human |
| IMMUNOGEN: | Human protein was used as the immunogen for the recombinant CD99 antibody. |
| TESTED APPLICATIONS: | Flow, IF, IHC-P |
| APPLICATIONS: | Flow Cytometry: 2-4 ug/million cells in 0.1ml Immunofluorescence: 2-4 ug/ml Immunohistochemistry (FFPE): 5-10 ug/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) Titering of the recombinant CD99 antibody may be required for optimal performance. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Specifications| PURIFICATION: | Protein A affinity chromatography |
| CLONALITY: | Monoclonal |
| ISOTYPE: | IgG, kappa |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
| CONCENTRATION: | 0.2 mg/mL |
| STORAGE CONDITIONS: | Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. |
| OFFICIAL SYMBOL: | CD99 |
| ALTERNATE NAMES: | CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 molecule, E2 antigen, HBA71 |
| GENE ID: | 4267 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher |
| BACKGROUND: | CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein. [Wiki] |
ANTIBODIES FOR RESEARCH USE ONLY.
For additional information, visit ProSci's Terms & Conditions Page.
