Recombinant CD8 Antibody [C8/1779R] Cat. No.: 33-815

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Recombinant full-length human CD8a protein was used as immunogen for this recombinant CD8 antibody.
TESTED APPLICATIONS: Flow, IF, IHC-P
APPLICATIONS: Flow Cytometry: 0.5-1 ug/million cells

Immunofluorescence: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD8 antibody to be titered up or down for optimal performance.

psi-iconProperties
PURIFICATION:Protein G affinity chromatography
CLONALITY:Monoclonal
ISOTYPE:IgG, k
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.2 mg/mL
STORAGE CONDITIONS:Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:CD8A
ALTERNATE NAMES:T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL
GENE ID:925
USER NOTE:Optimal dilutions for each application to be determined by the researcher
psi-iconBackground and References
BACKGROUND:CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

ANTIBODIES FOR RESEARCH USE ONLY.

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