| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human |
| IMMUNOGEN: | Recombinant full-length human CD8a protein was used as immunogen for this recombinant CD8 antibody. |
| TESTED APPLICATIONS: | Flow, IF, IHC-P |
| APPLICATIONS: | Flow Cytometry: 0.5-1 ug/million cells Immunofluorescence: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 min at RT The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD8 antibody to be titered up or down for optimal performance. |
Specifications| PURIFICATION: | Protein G affinity chromatography |
| CLONALITY: | Monoclonal |
| ISOTYPE: | IgG, k |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
| CONCENTRATION: | 0.2 mg/mL |
| STORAGE CONDITIONS: | Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. |
| OFFICIAL SYMBOL: | CD8A |
| ALTERNATE NAMES: | T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL |
| GENE ID: | 925 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher |
| BACKGROUND: | CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen. |
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