HOST SPECIES: | Rabbit |
SPECIES REACTIVITY: | Human |
IMMUNOGEN: | A full length recombinant human protein was used as the immunogen for the rabbit monoclonal CD31 antibody. |
TESTED APPLICATIONS: | IF, IHC |
APPLICATIONS: | Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Titering of the CD31 antibody may be required for optimal performance. 1. Staining of formalin-fixed tissues is enhance by boiling sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. |
PURIFICATION: | Protein A affinity |
CLONALITY: | Monoclonal |
ISOTYPE: | IgG, k |
CONJUGATE: | Unconjugated |
PHYSICAL STATE: | Liquid |
BUFFER: | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
CONCENTRATION: | 0.2 mg/mL |
STORAGE CONDITIONS: | Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. |
OFFICIAL SYMBOL: | PECAM1 |
ALTERNATE NAMES: | Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1 |
GENE ID: | 5175 |
USER NOTE: | Optimal dilutions for each application to be determined by the researcher |
BACKGROUND: | CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq] |
ANTIBODIES FOR RESEARCH USE ONLY.
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