| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human |
| IMMUNOGEN: | Recombinant protein was used as the immunogen for the Calponin antibody. |
| TESTED APPLICATIONS: | Flow, IF, IHC-P |
| APPLICATIONS: | Flow Cytometry: 0.5-1ug/10^6 cells Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1) Optimal dilution of the Calponin antibody should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min |
Specifications| PURIFICATION: | Protein A affinity chromatography |
| CLONALITY: | Monoclonal |
| ISOTYPE: | IgG, k |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
| CONCENTRATION: | 0.2 mg/mL |
| STORAGE CONDITIONS: | Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. |
| OFFICIAL SYMBOL: | CNN1 |
| ALTERNATE NAMES: | CNN1, Basic calponin, Calponin, Calponin-1, Calponins, basic, Calponin H1, smooth muscle, SMCC, Sm-Calp |
| GENE ID: | 1264 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher |
| BACKGROUND: | Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. |
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