HOST SPECIES: | Rabbit |
SPECIES REACTIVITY: | Human, Rat |
IMMUNOGEN: | A full length human protein was used as the immunogen for the recombinant Caldesmon antibody. |
TESTED APPLICATIONS: | Flow, IF, IHC-P |
APPLICATIONS: | Flow Cytometry: 0.5-1ug/10^6 cells Immunofluorescence: 1-2 ug/ml Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) Titering of the recombinant Caldesmon antibody may be required for optimal performance. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
PURIFICATION: | Protein A affinity chromatography |
CLONALITY: | Monoclonal |
ISOTYPE: | IgG, k |
CONJUGATE: | Unconjugated |
PHYSICAL STATE: | Liquid |
BUFFER: | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
CONCENTRATION: | 0.2 mg/mL |
STORAGE CONDITIONS: | Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. |
OFFICIAL SYMBOL: | CALD1 |
ALTERNATE NAMES: | Caldesmon, CDM, CALD1, CAD, CDM |
GENE ID: | 800 |
USER NOTE: | Optimal dilutions for each application to be determined by the researcher |
BACKGROUND: | Caldesmon (CAD, CALD, CALD1) is an actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). [UniProt] |
ANTIBODIES FOR RESEARCH USE ONLY.
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