HOST SPECIES: | Rabbit |
SPECIES REACTIVITY: | Human |
IMMUNOGEN: | Purified human protein used as the immunogen for the recombinant CA19-9 antibody. |
TESTED APPLICATIONS: | Flow, IF, IHC-P, WB |
APPLICATIONS: | Flow Cytometry: 2-4 ug/million cells in 0.1ml Immunofluorescence: 5-10 ug/ml Immunohistochemistry (FFPE): 5-10 ug/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) Optimal dilution of the recombinant CA19-9 antibody should be determined by the researcher. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
PURIFICATION: | Protein A affinity |
CLONALITY: | Monoclonal |
ISOTYPE: | IgG, k |
CONJUGATE: | Unconjugated |
PHYSICAL STATE: | Liquid |
BUFFER: | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
CONCENTRATION: | 0.2 mg/mL |
STORAGE CONDITIONS: | Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. |
USER NOTE: | Optimal dilutions for each application to be determined by the researcher |
BACKGROUND: | CA19-9, a carbohydrate epitope expressed on a high MW (>400kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. |
ANTIBODIES FOR RESEARCH USE ONLY.
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