Recombinant ACTH Antibody [r57] Cat. No.: 33-810

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: An N-terminal peptide (within AA 1-24) from the Synacthen/ACTH region of human POMC was used as the immunogen for this recombinant ACTH antibody.
TESTED APPLICATIONS: Flow, IF, IHC-P
APPLICATIONS: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT

The optimal dilution of the recombinant ACTH antibody for each application should be determined by the researcher.

psi-iconProperties
PURIFICATION:Protein G affinity chromatography
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.2 mg/mL
STORAGE CONDITIONS:Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:POMC
ALTERNATE NAMES:POMC, proopiomelanocortin, Precursor of MSH, POC, ACTH, CLIP, adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin, LPH, MSH, NPP
GENE ID:5443
USER NOTE:Optimal dilutions for each application to be determined by the researcher
psi-iconBackground and References
BACKGROUND:ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

ANTIBODIES FOR RESEARCH USE ONLY.

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