Rabbit Anti-Chicken IgY (H&L) Antibody (Alkaline Phosphatase) Cat. No.: XR-9180

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY:Chicken
IMMUNOGEN:Chicken IgY - whole molecule
CONJUGATE:Alkaline Phosphatase
TARGET SPECIES:Reacts with the heavy chain of chicken IgY.
TESTED APPLICATIONS:ELISA
APPLICATIONS:Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 mg of Chicken IgY in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product. (Optimal dilution should be determined by user).
psi-iconProperties
PURIFICATION:Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgY and Chicken Serum.
ISOTYPE:IgY
CONJUGATE:Alkaline Phosphatase
PHYSICAL STATE:Liquid
BUFFER:0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.1% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (BSA)
STORAGE CONDITIONS:Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY can be stored vial at 4 ˚C before opening. DO NOT FREEZE. This product is stable at 4 ˚C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
psi-iconBackground and References
REFERENCES:1) Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.

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