QSOX1 Antibody Cat. No.: 8171

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (76%)
IMMUNOGEN: QSOX1 antibody was raised against a 17 amino acid peptide near the center of human QSOX1.

The immunogen is located within amino acids 360 - 410 of QSOX1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: QSOX1 antibody can be used for detection of QSOX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: QSOX1 antibody is human and mouse reactive. QSOX1 antibody is predicted to not cross-react with QSOX2.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 65, 82 kDa

Observed: 66 kDa

psi-iconProperties
PURIFICATION:QSOX1 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:QSOX1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:QSOX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:QSOX1
ALTERNATE NAMES:Quiescin Q6 sulfhydryl oxidase 1, Sulfhydryl oxidase 1, Q6, QSCN6
ACCESSION NO.:NP_002817
PROTEIN GI NO.:13325075
GENE ID:5768
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The QSOX1 gene, also known as Quiescin Q6, is a fusion of two ancient genes: thioredoxin and ERV1. Its expression is induced as fibroblasts begin to exit the proliferative cycle and enter quiescence, suggesting that this gene plays an important role in growth regulation (1). The QSOX1 protein oxidizes sulfhydryl groups to form disulfide bonds in proteins. QSOX1 expression is induced by hypoxia (2) and appears to protect cells against oxidative stress-induced apoptosis (3).
REFERENCES:1) Coppock DL, Cina-Poppe D, and Gilleran S. The quiescin Q6 gene (QSCN6) is a fusion of two ancient gene families: thioredoxin and ERV1. Genomics 1998; 54:460-8.
2) Shi CY, Fan Y, Liu B, et al. HIF1 contributes to hypoxia-induced pancreatic cancer cells invasion via promoting QSOX1 expression. Cell Physiol. Biochem. 2013; 32:561-8.
3) Morel C, Adami P, Musard JF, et al. Involvement of sulfhydryl oxidase QSOX1 in the protection of cells against oxidative stress-induced apoptosis. Exp. Cell Res. 2007; 313:3971-82.

ANTIBODIES FOR RESEARCH USE ONLY.

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