PUMA Fragment Recombinant Protein Cat. No.: 95-102

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psi-iconSpecifications
SPECIES:Human
SOURCE SPECIES:E. coli
SEQUENCE:aa. 76 - 170
FUSION TAG:Fusion Partner: His-tag and strepII-tag at N-terminus
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:This recombinant protein can be used for WB and ELISA. For research use only.
BIOLOGICAL ACTIVITY: N/A
psi-iconProperties
PURITY:~95%
PREDICTED MOLECULAR WEIGHT:15 kDa (Calculated)
PHYSICAL STATE:Liquid
BUFFER:1X PBS containing 0.1% SDS and 0.02% NaN3
STORAGE CONDITIONS:Store in working aliquots at -70˚C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
psi-iconAdditional Info
ALTERNATE NAMES:PUMA Antibody : JFY1, PUMA, JFY-1
ACCESSION NO.:Q9BXH1
PROTEIN GI NO.:56748610
OFFICIAL SYMBOL:BBC3
GENE ID:27113
psi-iconBackground and References
BACKGROUND:Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA- and PUMA- (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. This PUMA recombinant protein is recognized by several ProSci PUMA monoclonal antibodies, but not the two polyclonal antibodies (PSI # 3041 and 3043).
REFERENCES:1) Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.
2) Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.
3) Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.

FOR RESEARCH USE ONLY.

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