PUMA Antibody [10C5G1] Cat. No.: PM-4527

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human, Rat
IMMUNOGEN: Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 95-102 - PUMA Recombinant Protein

psi-iconProperties
PURIFICATION:PUMA Monoclonal Antibody is immunoaffinity chromotography purified IgG.
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PUMA Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:PUMA monoclonal antibody can be stored at -20˚C, stable for one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:BBC3
ALTERNATE NAMES:PUMA Antibody [10C5G1] : JFY1, PUMA, JFY-1
ACCESSION NO.:Q9BXH1
PROTEIN GI NO.:56748610
GENE ID:27113
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα and PUMAβ. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
REFERENCES:1) Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.
2) Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.
3) Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.

ANTIBODIES FOR RESEARCH USE ONLY.

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