Specifications
- HOST SPECIES: Goat
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Human, Cow
- IMMUNOGEN: The immunogen for this antibody is: C-RHLSLGSRHRGDK
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave bands at approx 75kDa and 50kDa in HeLa nuclear lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 60.5kDa according to NP_001073939.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: ZNF697
- ADDITIONAL NAMES: ZNF697, zinc finger protein 697, MGC45731, OTTHUMP00000015145
- Protein Accession Number: NP_001073939.1
- PROTEIN GI NUMBER: 122937333
- NCBI GENE ID NUMBER: 90874
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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