- Trial Size
ZIP1 Antibody
CATALOG NUMBER: 6081- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (88%)
- IMMUNOGEN: ZIP1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human ZIP1.
The immunogen is located within amino acids 40 - 90 of ZIP1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: ZIP1 antibody can be used for detection of ZIP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: At least two isoforms of ZIP1 are known to exist; this antibody will detect both isoforms. ZIP1 antibody is predicted not to cross-react with other ZIP family proteins.
- POSITIVE CONTROL 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-401 - Human Kidney Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 13, 36 kDa
Observed: 38 kDa
Properties
- PURIFICATION: ZIP1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: ZIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: ZIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: SLC39A1
- ADDITIONAL NAMES: ZIP1 Antibody: ZIP1, ZIRTL, IRT1, ZIP1, CGI-08, CGI-71, Zinc transporter ZIP1, Solute carrier family 39 member 1, ZIP-1
- Protein Accession Number: NP_055252
- PROTEIN GI NUMBER: 21361423
- NCBI GENE ID NUMBER: 27173
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- ZIP1 Antibody: ZIP1, also known as ZIRTL (zinc-iron regulated transporter-like), is the first mammalian member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP1 expression is markedly downregulated in a number of cancerous tissues and is thought to function as a tumor suppressor gene in prostate cancer. More recent studies have shown that overexpression of ZIP1 and concomitant increased levels of intracellular zinc in PC-3 cells cause a significant inhibition of NF-κB, leading to down-regulation of the antiapoptotic protein Bcl-2, Bcl-XL, and the apoptosis inhibitor XIAP, reducing the malignant potential of prostate cancer cells in vitro and in vivo.
- 1: Lioumi M, Ferguson CA, Sharpe PT, et al. Isolation and characterization of human and mouse ZIRTL, a member of the IRT1 family of transporters, mapping within the epidermal differentiation complex. Genomics 1999; 62:272-80.
- 2: Eide DJ. The SLC39 family of metal ion transporters. Pflugers Arch. 2004; 447:796-800.
- 3: Taylor KM and Nicohlson RI. The LZT proteins; the LIV-1 subfamily of zinc transporters. Biochim. Biophys. Acta. 2003; 1611:16-30.
- 4: Costello LC and Franklin RB. The clinical relevance of the metabolism of prostate cancer; zinc and tumor suppression: connecting the dots. Mol. Cancer. 2006; 5:17.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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