- Trial Size
ZFX Antibody
CATALOG NUMBER: 6811- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
Powered by BiozSpecifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (94%)
- IMMUNOGEN: ZFX antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFX.
The immunogen is located within the last 50 amino acids of ZFX. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: ZFX antibody can be used for detection of ZFX by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: ZFX antibody is human, mouse and rat reactive. Multiple isoforms of ZFX are known to exist. ZFX antibody will detect isoform 1 and 2 of ZFX.
- POSITIVE CONTROL 1: Cat. No. 1224 - Daudi Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1223 - Caco-2 Cell Lysate
- POSITIVE CONTROL 3: Cat. No. 11-801 - Human Small Intestine Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 89, 63 kDa
Observed: 63 kDa
Properties
- PURIFICATION: ZFX Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: ZFX Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: ZFX antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ZFX
- ADDITIONAL NAMES: ZFX Antibody: ZNF926, Zinc finger X-chromosomal protein
- Protein Accession Number: NP_003401
- PROTEIN GI NUMBER: 71061446
- NCBI GENE ID NUMBER: 7543
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- ZFX Antibody: The X-linked zinc finger protein ZFX is a member of the Kruppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny.
- 1: Schneider-Gadicke A, Beer-Romero P, Brown LG, et al. Putative transcription activator with alternative isoforms encoded by human ZFX gene. Nature 1989; 342:708-11.
- 2: Galan-Caridad JM, Harel S, Arenzana TL, et al. Zfx controls the self-renewal of embryonic and hematopoietic stem cells. Cell 2007; 129:345-57.
- 3: Arenzana TL, Smith-Raska MR, and Reizis B. Transcription factor Zfx controls BCR-induced proliferation and survival of B lymphocytes. Blood 2009; 113:5857-67
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.
New & Featured Products
![HIV-1 p24 Antibody [10A2] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-10A2_WB_PM-7371.gif){kind=small}
HIV-1 p24 Antibody [10A2] (biotin)
CATALOG NUMBER: PM-7371-biotin
![HIV-1 p24 Antibody [9D2F6] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-9D2F6_WB_PM-7369.gif){kind=small}
HIV-1 p24 Antibody [9D2F6] (biotin)
CATALOG NUMBER: PM-7369-biotin
![HIV-1 p24 Antibody [8D7E11] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-8D7E11_WB_PM-7367.gif){kind=small}
HIV-1 p24 Antibody [8D7E11] (biotin)
CATALOG NUMBER: PM-7367-biotin