- Trial Size
ZBTB7A Antibody
CATALOG NUMBER: 6127- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (94%), Chicken: (88%)
- IMMUNOGEN: ZBTB7A antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human ZBTB7A.
The immunogen is located within the first 50 amino acids of ZBTB7A. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: ZBTB7A antibody can be used for detection of ZBTB7A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: At least two isoforms of ZBTB7A are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.
- POSITIVE CONTROL 1: Cat. No. 1316 - Human Ovary Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 11-201 - Human Ovary Tissue Slide
Properties
- PURIFICATION: ZBTB7A Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: ZBTB7A Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: ZBTB7A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ZBTB7A
- ADDITIONAL NAMES: ZBTB7A Antibody: LRF, FBI1, FBI-1, ZBTB7, ZNF857A, pokemon, LRF, Zinc finger and BTB domain-containing protein 7A, Factor binding IST protein 1
- Protein Accession Number: AAH84568
- PROTEIN GI NUMBER: 7705375
- NCBI GENE ID NUMBER: 51341
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- ZBTB7A Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB7A, also known as LRF, was initially identified as a potential target of the LAZ-3/BCL-6 oncogene. Later reports demonstrated that it could bind to the wild-type IST (inducer of short transcripts) elements of HIV-1 long terminal repeats (LTRs) and stimulate Tat activity on the HIV-1 LTR. More recently it ZBTB7A has been recognized as a proto-oncogene whose overexpression contributes to malignancy in breast cancer and functions as a co-repressor of the androgen receptor in prostate cancer cells.
- 1: Filion GJP, Zhenilo S, Salozhin S, et al. A family of zinc finger proteins that bind methylated DNA and repress transcription. Mol. Cell. Biol.2006; 26:169-81.
- 2: Davies JM, Hawe N, Kabarowski J, et al. Novel BTB/POZ domain zinc-finger protein, LRF, is a potential target of the LAZ-3/BCL-6 oncogene. Oncogene1999; 18:365-75.
- 3: Pendergrast PS, Wang C, Hernandez N, et al. FBI-1 can stimulate HIV-1 Tat activity and is targeted to a novel subnuclear domain that includes the Tat-P-TEFb-containing nuclear speckles. Mol. Cell. Biol.2002; 13:915-29.
- 4: Qu H, Qu D, Chen F, et al. ZBTB7 overexpression contributes to malignancy in breast cancer. Cancer Invest.2010; 28:672-8.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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