- Trial Size
ZBRK1 Antibody
CATALOG NUMBER: 4817- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: ZBRK1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human ZBRK1.
The immunogen is located within amino acids 410 - 460 of ZBRK1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: ZBRK1 antibody can be used for detection of ZBRK1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1288 - A20 Cell Lysate
Properties
- PURIFICATION: ZBRK1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: ZBRK1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: ZBRK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ZNF350
- ADDITIONAL NAMES: ZBRK1 Antibody: ZFQR, ZBRK1, Zinc finger protein 350, KRAB zinc finger protein ZFQR
- Protein Accession Number: NP_067645
- PROTEIN GI NUMBER: 59710107
- NCBI GENE ID NUMBER: 59348
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- ZBRK1 Antibody: ZBRK1 is a member of the Kruppel-associated box-zinc finger protein (KRAB-ZFP) protein family of transcriptional repressors. ZBRK1 has been shown to not only be up-regulated in senescent fibroblasts but also interacts with BRCA1, a transcriptional regulator with tumor suppressor activity. ZBRK1 binds to a specific sequence within the GADD45 gene, supporting the assembly of a nuclear complex containing BRCA1 and repressing its transcription. Recent experiments demonstrate that ZBRK1 can homo-oligomerize in vitro and in vivo through a C-terminal transcriptional repression domain (CTRD), and this oligomerization facilitates the ZBRK1-directed transcriptional repression through ZBRK1 response elements. These results suggest that higher order ZBRK1 oligomers may assemble through both protein-DNA and CTRD-dependent protein-protein interactions.
- 1: Zheng L, Pan H, Li S, et al. Sequence-specific transcription corepressor function for BRCA1 through a novel Zinc finger protein, ZBRK1. Mol. Cell2000; 6:757-68.
- 2: Collins T, Stone JR, and Williams AJ. All in the family: the BTB/POZ, KRAB, and SCAN domains. Mol. Cell. Biol.2001; 21:3609-15.
- 3: Tan W, Kim S, and Boyer TG. Tetrameric oligomerization mediates transcriptional repression by the BRCA1-dependent Kruppel-associated Box-Zinc finger protein ZBRK1. J. Biol. Chem.2004; 279:55153-60.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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