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Home / Human / ZAP-70 Antibody [ZTP70-1]
ZAP-70 Antibody [ZTP70-1]
ZAP-70 Antibody [ZTP70-1]

ZAP-70 Antibody [ZTP70-1]

CATALOG NUMBER: 33-988
  • Clonality: Monoclonal
  • Tested Applications: IF, IHC-P
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: Recombinant full-length human protein was used as the immunogen for the ZAP-70 antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: IF, IHC-P
  • APPLICATION NOTE: Immunofluorescence: 1-2 ug/ml
    Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)
    Titering of the ZAP-70 antibody may be required for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG2a, kappa
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: ZAP70
  • ADDITIONAL NAMES: Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, ZAP70, SRK
  • NCBI GENE ID NUMBER: 7535
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • Tyrosine-protein kinase ZAP70 is a tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, it phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. [UniProt]

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-988

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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