- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human XBP1.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: XBP1 antibody can be used for detection of XBP1 by ELISA at 1:1562500. XBP1 antibody can be used for detection of XBP1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- POSITIVE CONTROL 1: Cat. No. 1205 - Jurkat Cell Lysate
- PREDICTED MOLECULAR WEIGHT: 29 kDa
- PURIFICATION: Antibody is purified by peptide affinity chromatography method.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For short periods of storage (days) store at 4°C. For longer periods of storage, store XBP1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
- NCBI OFFICIAL SYMBOL: XBP1
- ADDITIONAL NAMES: XBP1, TREB5, XBP2, XBP-1
- Protein Accession Number: NP_005071
- PROTEIN GI NUMBER: 14110395
- NCBI GENE ID NUMBER: 7494
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- XBP1 is a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. XBP1 is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator.This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1 (S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1 (U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1 (S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
- 1: Davies, M.P., (2008) Int. J. Cancer 123 (1), 85-88.
- FOR RESEARCH USE ONLY
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- Disclaimer: This product is for research use only.
CATALOG NUMBER: 28-891
- Size: 100 ul
- List Price: $475.00
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