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XBP-1 Antibody [3H1G4]XBP-1 Antibody [3H1G4]XBP-1 Antibody [3H1G4]
XBP-1 Antibody [3H1G4]
XBP-1 Antibody [3H1G4]
XBP-1 Antibody [3H1G4]
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XBP-1 Antibody [3H1G4]

CATALOG NUMBER: PM-4935
  • Clonality: Monoclonal
  • Tested Applications: ELISA, ICC, IF, WB
  • Host Species: Mouse
  • Species Reactivity: Human, Mouse, Rat
  • Conjugate: Unconjugated
  • Size: 0.02 mg | 0.1 mg

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human, Mouse, Rat
  • IMMUNOGEN: Mouse monoclonal XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: ELISA, ICC, IF, WB
  • APPLICATION NOTE: XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
    Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
  • POSITIVE CONTROL 1: Cat. No. 95-109 - XBP-1 Recombinant Protein
  • POSITIVE CONTROL 2: Cat. No. 17-011 - Hep G2 Cell Slide

Properties

  • PURIFICATION: XBP-1 Monoclonal Antibody is Protein A purified.
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1
  • PHYSICAL STATE: Liquid
  • BUFFER: XBP-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
  • CONCENTRATION: 1 mg/mL
  • STORAGE CONDITIONS: XBP-1 monoclonal antibody can be stored at -20°C, stable for one year.

Additional Info

  • NCBI OFFICIAL SYMBOL: XBP1
  • ADDITIONAL NAMES: XBP-1 Antibody [3H1G4] : XBP2, TREB5, XBP-1
  • Protein Accession Number: BAB82982
  • PROTEIN GI NUMBER: 18148382
  • NCBI GENE ID NUMBER: 7494
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • XBP-1 Monoclonal Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
  • 1: Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.
  • 2: Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.
  • 3: Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.
  • 4: Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: PM-4935

  • Size: 0.02 mg | 0.1 mg
  • List Price: $89.00 – $459.00
Datasheet
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