Specifications
- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- IMMUNOGEN: The immunogen for this antibody is: QDPASTCVPEPASQH
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human Kidney, Spleen and Testis lysates (calculated MW of 54.3kDa according to NP_000369.3). Recommended concentration: 1-3ug/ml.
- SPECIFICITY: This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3).
- POSITIVE CONTROL 1: Cat. No. 1306 - Human Spleen Tissue Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: WT1
- ADDITIONAL NAMES: WT1, Wilms tumor 1, GUD, WAGR, WIT-2, WT33
- Protein Accession Number: NP_000369.3,NP_077742.2,NP_077743.2,NP_077744.3
- PROTEIN GI NUMBER: 65507714
- NCBI GENE ID NUMBER: 7490
Background
- 1: Rao MK, Pham J, Imam JS, MacLean JA, Murali D, Furuta Y, Sinha-Hikim AP, Wilkinson MF. Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis. Genes Dev. 2006 Jan 15;20(2):147-52.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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