Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rabbit
- IMMUNOGEN: This WNT5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-213 amino acids from the Central region of human WNT5A.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IHC-P, WB
- APPLICATION NOTE: For WB starting dilution is: 1:500
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50 - PREDICTED MOLECULAR WEIGHT: 42 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: WNT5A
- ADDITIONAL NAMES: Protein Wnt-5a, WNT5A
- Protein Accession Number: P41221
- PROTEIN GI NUMBER: 212276478
- NCBI GENE ID NUMBER: 7474
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The WNT gene family consists of structurally related geneswhich encode secreted signaling proteins. These proteins have beenimplicated in oncogenesis and in several developmental processes,including regulation of cell fate and patterning duringembryogenesis. This gene is a member of the WNT gene family. Itencodes a protein which shows 98%, 98% and 87% amino acid identityto the mouse, rat and the xenopus Wnt5A protein, respectively. Theexperiments performed in Xenopus laevis embryos identified thathuman frizzled-5 (hFz5) is the receptor for the Wnt5A ligand andthe Wnt5A/hFz5 signaling mediates axis induction. [provided byRefSeq].
- 1: He, X., et al. Science 275(5306):1652-1654(1997)
- 2: Danielson, K.G., et al. J. Biol. Chem. 270(52):31225-31234(1995)
- 3: Smolich, B.D., et al. Mol. Biol. Cell 4(12):1267-1275(1993)
- 4: Clark, C.C., et al. Genomics 18(2):249-260(1993)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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