Wnt4 Antibody
CATALOG NUMBER: 45-172- Clonality: Polyclonal
- Tested Applications: ELISA, IF, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat
- IMMUNOGEN: The immunogen for this antibody is: C-SNWLYLAKLSSVGS
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40+35kDa band observed in lysates of cell line Jurkat (calculated MW of 39.1kDa according to NP_110388.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: U937 cell lysate.Immunofluorescence: Strong expression of the protein seen in the vesicles of HeLa and MCF7 cells. Recommended concentration: 10ug/ml.
- POSITIVE CONTROL 1: Cat. No. 1316 - Human Ovary Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1313 - Human Testis Tissue Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: WNT4
- ADDITIONAL NAMES: WNT4, wingless-type MMTV integration site family, member 4, WNT-4, WNT-4 protein precursor, RP1-224A6.7, SERKAL, WNT-4 protein
- Protein Accession Number: NP_110388.2
- PROTEIN GI NUMBER: 17402922
- NCBI GENE ID NUMBER: 54361
Background
- 1: Huguet EL, McMahon JA, McMahon AP, Bicknell R, Harris AL. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell. Cancer Res. 1994 May 15;54(10):2615-21.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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