- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Human WNT-3a specific antibody was purified by affinity chromatography and then biotinylated.
- CONJUGATE: Biotin
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: ELISA:
To detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human WNT-3a as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.
To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: WNT-3a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
- NCBI OFFICIAL SYMBOL: WNT3A
- ADDITIONAL NAMES: Protein Wnt-3a
- Protein Accession Number: P56704
- PROTEIN GI NUMBER: 20532424
- NCBI GENE ID NUMBER: 89780
- USER NOTE: Centrifuge vial prior to opening.
- Wnt3a is a secreted ligand for members of the Frizzled family of receptors. It is one of the closely-related Wnt family and has multiple roles in development and cell fate.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: This product is for research use only.
CATALOG NUMBER: 38-178
- Size: 0.05 mg
- List Price: $365.00
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