WNT-1 Antibody (biotin)
CATALOG NUMBER: 38-176- Clonality: Polyclonal
- Tested Applications: E, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Biotin
- Size: 0.05 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-1. Human WNT-1 specific antibody was purified by affinity chromatography and then biotinylated.
- CONJUGATE: Biotin
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: ELISA:
Sandwich:
To detect hWNT-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human WNT-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1.
Western Blot:
To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: WNT-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: WNT1
- ADDITIONAL NAMES: INT1, OI15, BMND16, INT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog,
- Protein Accession Number: P04628
- PROTEIN GI NUMBER: 139743
- NCBI GENE ID NUMBER: 7471
- USER NOTE: Centrifuge vial prior to opening.
Background
- Wnt1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta catenine. Mature human Wnt1 is a glycosylated protein containing 343 amino acid residues.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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