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- Trial Size
VISTA Antibody [8E11]
CATALOG NUMBER: RF16073- Clonality: Monoclonal
- Tested Applications: ELISA, Flow, ICC, IF, IHC-P
- Host Species: Mouse
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
Powered by BiozSpecifications
- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human
- IMMUNOGEN: VISTA antibody was raised against the extracellular domain of human VISTA.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Flow, ICC, IF, IHC-P
- APPLICATION NOTE: VISTA antibody can be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL. Flow cytometry at 10 μg/ml.
Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1331 - Human Lymphoma Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1306 - Human Spleen Tissue
- PREDICTED MOLECULAR WEIGHT: Predicted: 31 kDa
Observed: 47 kDa
Properties
- PURIFICATION: VISTA Antibody is supplied as protein A purified IgG1.
- CLONALITY: Monoclonal
- ISOTYPE: IgG1
- PHYSICAL STATE: Liquid
- BUFFER: VISTA Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: VISTA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: VSIR
- ADDITIONAL NAMES: VISTA Antibody: VISTA molecule, VSIR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1alpha, VISTA, C10orf54, chromosome 10 open reading frame 54, PD-1H, V-set immunoregulatory receptor, V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation, Chromosome 10 Open Reading Frame 54
- Protein Accession Number: NP_071436
- PROTEIN GI NUMBER: 64115
- NCBI GENE ID NUMBER: 64115
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.
- 1: Mayya V., et al . Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46.
- 2: Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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