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Home / Cancer / Vimentin Antibody [VIM66-1]
Vimentin Antibody [VIM66-1]Vimentin Antibody [VIM66-1]
Vimentin Antibody [VIM66-1]
Vimentin Antibody [VIM66-1]

Vimentin Antibody [VIM66-1]

CATALOG NUMBER: 33-861
  • Clonality: Monoclonal
  • Tested Applications: IHC, WB
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: Full length human recombinant protein was used as the immunogen for the Vimentin antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: IHC, WB
  • APPLICATION NOTE: Western blot: 1-2 ug/ml
    IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)
    Prediluted format : incubate for 30 min at RT (2)
    Titering of the Vimentin antibody may be required for optimal performance.

    1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1, k
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: VIM
  • ADDITIONAL NAMES: Vimentin, VIM
  • NCBI GENE ID NUMBER: 7431
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-861

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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