Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Mouse, Pig, Rat
- IMMUNOGEN: This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-181 amino acids from the Central region of human Vimentin.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IHC-P, WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50 - PREDICTED MOLECULAR WEIGHT: 54 kDa
Properties
- PURIFICATION: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: VIM
- ADDITIONAL NAMES: Vimentin, VIM
- Protein Accession Number: P08670
- PROTEIN GI NUMBER: 55977767
- NCBI GENE ID NUMBER: 7431
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
- 1: Whipple,R.A.,Cancer Res. 68 (14), 5678-5688 (2008)
- 2: Garcia-Verdugo,I.,Biochemistry 47 (18), 5127-5138 (2008)
- 3: Merdes,A., J. Cell Biol. 115 (2), 397-410 (1991)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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