Vimentin Antibody
CATALOG NUMBER: 43-649- Clonality: Polyclonal
- Tested Applications: ELISA, Flow, IF, WB
- Host Species: Goat
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
- IMMUNOGEN: The immunogen for this antibody is: C-QVINETSQHHDDLE
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Flow, IF, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60kDa band in Rat Ovary lysates (calculated MW of 53.7kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1). Recommended concentration: 0.1-2ug/ml.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended concentration: 5ug/ml.
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- PREDICTED MOLECULAR WEIGHT: Approx 55kDa
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: Vimentin
- ADDITIONAL NAMES: VIM, vimentin, FLJ36605
- Protein Accession Number: NP_003371.2
- PROTEIN GI NUMBER: 55977767
- NCBI GENE ID NUMBER: 7431
Background
- 1: Maraina CH, Nurdayana AK, Rusni D, Azwany Y.Diagnostic value of anti-modified citrullinated vimentin in rheumatoid arthritis.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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