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VIM Antibody [SPM576]
VIM Antibody [SPM576]

VIM Antibody [SPM576]

CATALOG NUMBER: 34-182
  • Clonality: Monoclonal
  • Tested Applications: Flow, IF, IHC, WB
  • Host Species: Mouse
  • Species Reactivity: Bovine, Cat, Chicken, Dog, Goat, Human, Pig
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Bovine, Cat, Chicken, Dog, Goat, Human, Pig
  • IMMUNOGEN: Human recombinant protein was used as the immunogen for this VIM antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, IF, IHC, WB
  • APPLICATION NOTE: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
    Immunofluorescence: 1-2 ug/ml
    Western blot: 0.25-0.5 ug/ml
    Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)
    The optimal dilution of the VIM antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

  • SPECIFICITY: Does not react with mouse and rat

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: VIM
  • ADDITIONAL NAMES: Vimentin, VIM
  • NCBI GENE ID NUMBER: 7431
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • This mAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 34-182

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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