- Trial Size
VEZT Antibody
CATALOG NUMBER: 7959- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: VEZT antibody was raised against a 17 amino acid peptide near the center of human VEZT.
The immunogen is located within amino acids 480 - 530 of VEZT. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: VEZT antibody can be used for detection of VEZT by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. - SPECIFICITY: VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist.
- POSITIVE CONTROL 1: Cat. No. 1210 - HEK293 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 63, 68, 78, 81, 83, 86 kDa
Observed: 60 kDa
Properties
- PURIFICATION: VEZT antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: VEZT antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: VEZT antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: VEZT
- ADDITIONAL NAMES: VEZT Antibody: VEZATIN, Vezatin
- Protein Accession Number: NP_060069
- PROTEIN GI NUMBER: 155030244
- NCBI GENE ID NUMBER: 55591
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Vezatin (VEZT) is a transmembrane protein which localizes to adherens junctions and binds to myosin VIIA and the cadherin-catenins complex (1). Expression of this gene in gastric cancer tissues is decreased due to hypermethylation of the promoter. Expression of this gene may also be inhibited by binding of a specific microRNA to a target sequence in the 3' UTR of the transcripts (2). VEZT has also been shown to be required for morphogenesis of the preimplantation mouse embryo (3).
- 1: Kussel-Andermann P, El-Amraoui A, Safieddine S, et al. Vezatin, a novel transmembrane protein, bridges myosin VIIA to the cadherin-catenins complex. EMBO J. 2000; 19:6020-9.
- 2: Guo X, Jing C, Zhang L, et al. Down-regulation of VEZT gene expression in human gastric cancer involves promoter methylation and miR-43c. Biochem. Biophys. Res. Commun. 2011; 404:622-7.
- 3: Hyenne V, Louvet-Vallee S, El-Amraoui A, et al. Vezatin, a protein associated to adherins junctions, is required for mouse blastocyst morphogenesis. Dev. Biol. 2005; 287:180-91.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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