Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVaspin. Human Vaspin specific antibody was purified by affinity chromatography and then biotinylated.
- CONJUGATE: Biotin
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: ELISA:
Sandwich:
To detect hVaspin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Vaspin as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.
Western Blot:
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Vaspin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: SERPINA12
- ADDITIONAL NAMES: OL-64Serpin A12, OL-64, Vaspin
- Protein Accession Number: Q8IW75
- PROTEIN GI NUMBER: 74728144
- NCBI GENE ID NUMBER: 145264
- USER NOTE: Centrifuge vial prior to opening.
Background
- Vaspin (serpina12) is a adipocytokine expressed predominantly in visceral white adipose tissues. It has three beta sheets, nine alpha helices, and one central loop, which are distinctive structural features of Serpin family members. The
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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