- Trial Size
UVRAG Antibody
CATALOG NUMBER: 7947- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: UVRAG antibody was raised against an 18 amino acid peptide near the carboxy terminus of human UVRAG.
The immunogen is located within amino acids 490 - 540 of UVRAG. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: UVRAG antibody can be used for detection of UVRAG by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: UVRAG antibody is human and mouse reactive.
- POSITIVE CONTROL 1: Cat. No. 1402 - Mouse Lung Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-101 - Human Lung Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 77 kDa
Observed: 75 kDa
Properties
- PURIFICATION: UVRAG antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: UVRAG antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: UVRAG antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: UVRAG
- ADDITIONAL NAMES: UVRAG Antibody: p63, DHTX, VPS38, UV radiation resistance-associated gene protein, p63
- Protein Accession Number: NP_003360
- PROTEIN GI NUMBER: 21687212
- NCBI GENE ID NUMBER: 7405
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The class III type phosphoinositide 3-kinase (PI3KC3) / Vps34 regulates vacuolar trafficking as well as autophagy (1). UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (2). UVRAG is highly expressed in brain, lung, kidney and liver and contains one C2 domain, which is involved in calcium-dependent phospholipid binding. Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (3). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (4,5).
- 1: Stack JH, DeWald DB, Takegawa K, et al. Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. J. Cell Biol. 1995; 129:321-34.
- 2: Liang C, Feng P, Ku B, et al. Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG. Nat. Cell Biol. 2006; 8:688-99.
- 3: Liang C, Lee JS, Inn KS, et al. Beclin1-binding UVRAG targets the class C Vps complex to coordinate autophagosome maturation and endocytic trafficking. Nat. Cell Biol. 2008; 10:776-87.
- 4: Ionov Y, Nowak N, Perucho M, et al. Manipulation of nonsense mediated decay identifies gene mutations in colon cancer Cells with microsatellite instability. Oncogene 2004; 23:639-45.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.
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