Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine
- IMMUNOGEN: This USP21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-436 amino acids from the C-terminal region of human USP21.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IHC-P, WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100 - PREDICTED MOLECULAR WEIGHT: 63 kDa
Properties
- PURIFICATION: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: USP21
- ADDITIONAL NAMES: Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23
- Protein Accession Number: Q9UK80
- PROTEIN GI NUMBER: 10720334
- NCBI GENE ID NUMBER: 27005
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- USP21 is a ubiquitin-specific protease, an enzyme that removes ubiquitin from ubiquitinated proteins. The encoded protein belongs to the C19 peptidase family, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. This protein has been reported to be capable of removing NEDD8 from NEDD8 conjugates.
- 1: Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
- 2: Gong, L., et al., J. Biol. Chem. 275(19):14212-14216 (2000).
- 3: Hillier, L.D., et al., Genome Res. 6(9):807-828 (1996).
- 4: Smith, T.S., et al., Biochim. Biophys. Acta 1490 (1-2), 184-188 (2000).
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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