- Trial Size
USP10 Antibody
CATALOG NUMBER: 5803- Clonality: Polyclonal
- Tested Applications: ELISA, ICC, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (80%), Rat: (91%), Chicken: (89%)
- IMMUNOGEN: USP10 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human USP10.
The immunogen is located within amino acids 30 - 80 of USP10. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, ICC, IF, WB
- APPLICATION NOTE: USP10 antibody can be used for detection of USP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1205 - Jurkat Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-005 - Jurkat Cell Slide
Properties
- PURIFICATION: USP10 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: USP10 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: USP10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: USP10
- ADDITIONAL NAMES: USP10 Antibody: UBPO, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10
- Protein Accession Number: NP_005144
- PROTEIN GI NUMBER: 119220605
- NCBI GENE ID NUMBER: 9100
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- USP10 Antibody: USP10, also known as ubiquitin specific peptidase 10, belongs to the ubiquitin-specific protease family of cysteine proteases. USP10 functions to catalyze the cleavage of ubiquitin from ubiquitin-conjugated protein substrates such as p53/TP53, SNX3 and CFTR. USP10 has been identified as a subunit of DNA-bound androgen receptor (AR) complexes and may play a role in the activity of the DNA-bound androgen receptor complex. USP10 also acts as an essential regulator of p53/TP53 stability and is thought to function as a tumor suppressor.
- 1: D'Andrea A and Pellman D. Deubiquitinating enzymes: a new class of biological regulators. Crit. Rev. Biochem. Mol. Biol.1998; 33:337-52.
- 2: Bomberger JM, Barnaby RL, and Stanton BA. The deubiquitinating enzyme USP10 regulates the endocytic recycling of CFTR in airway epithelial cells. Channels2010; 4(3).
- 3: Faus H, Meyer HA, Huber M, et al. The ubiquitin-specific protease USP10 modulates androgen receptor function. Mol. Cell. Endocrinol.2005; 245:138-46.
- 4: Yuan J, Luo K, Zhang L, et al. USP10 regulates p53 localization and stability by deubiquitinating p53. Cell 2010; 140:384-96.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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