UGT8 Antibody
CATALOG NUMBER: 42-832- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Goat
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Cow
- IMMUNOGEN: The immunogen for this antibody is: C-NGKYKRNGHIK
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:In transfected MCF7 transiently expressing Human UGT8 a band of approx. 65kDa is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5kDa according to NP_003351.2. Recommended concentration, 1-3ug/ml.Preliminary experiments gave an approx 50kDa band in Human -Brain lysates and 48KDa in Rat Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.5kDa according to NP_003351.2. The observed band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
- SPECIFICITY: Reported variants represent identical protein: NP_001121646.1, NP_003351.2.
- POSITIVE CONTROL 1: Cat. No. 1303 - Human Brain Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1461 - Rat Heart Tissue Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: UGT8
- ADDITIONAL NAMES: UGT8, UDP glycosyltransferase 8, CGT, UGT4, 2-hydroxyacylsphingosine 1-beta-galactosyltransferase, UDP-galactose ceramide galactosyltransferase, UDP-galactose-ceramide galactosyltransferase, ceramide UDP-galactosyltransferase, cerebroside synthase, uridine diphosphate glycosyltransferase 8
- Protein Accession Number: NP_003351.2
- PROTEIN GI NUMBER: 40254471
- NCBI GENE ID NUMBER: 7368
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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