Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Dog, Human, Mouse, Rat, Zebrafish
- IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2I.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: UBE2I antibody can be used for detection of UBE2I by ELISA at 1:312500. UBE2I antibody can be used for detection of UBE2I by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- POSITIVE CONTROL 1: Cat. No. 1205 - Jurkat Cell Lysate
- PREDICTED MOLECULAR WEIGHT: 17 kDa, 18 kDa, 18 kDa, 18 kDa
Properties
- PURIFICATION: Antibody is purified by protein A chromatography method.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For short periods of storage (days) store at 4°C. For longer periods of storage, store UBE2I antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: UBE2I
- ADDITIONAL NAMES: UBE2I, C358B7.1, P18, UBC9
- Protein Accession Number: NP_003336
- PROTEIN GI NUMBER: 4507785
- NCBI GENE ID NUMBER: 7329
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2I is a member of the E2 ubiquitin-conjugating enzyme family.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
- 1: Zhu, S., (2006) Exp. Cell Res. 312 (7), 1042-1049.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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