TWEAK Antibody
CATALOG NUMBER: XP-5290- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTWEAK (human TWEAK).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: ELISA:
To detect hTWEAK by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTWEAK.
Sandwich:
To detect hTWEAK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TWEAK (XP-5290Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTWEAK.
Western Blot:
To detect hTWEAK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTWEAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions..
Properties
- PURIFICATION: Anti-hTWEAK specific antibody was purified by affinity chromatography employing immobilized hTWEAK matrix.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: TWEAK antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: TNFSF12
- ADDITIONAL NAMES: APO3L, DR3LG, TWEAK, APO3L, UNQ181/PRO207, Tumor necrosis factor ligand superfamily member 12, APO3 ligand
- Protein Accession Number: O43508
- PROTEIN GI NUMBER: 21362987
- NCBI GENE ID NUMBER: 8742
- USER NOTE: Centrifuge vial prior to opening.
Background
- TWEAK is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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