- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: TSHZ3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TSHZ3.
The immunogen is located within amino acids 60 - 110 of TSHZ3.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: TSHZ3 antibody can be used for detection of TSHZ3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
- POSITIVE CONTROL 1: Cat. No. 1403 - Mouse Brain Tissue Lysate
- PURIFICATION: TSHZ3 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TSHZ3 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TSHZ3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: TSHZ3
- ADDITIONAL NAMES: TSHZ3 Antibody: TSH3, ZNF537, KIAA1474, TSH3, Teashirt homolog 3, Zinc finger protein 537
- Protein Accession Number: NP_065907
- PROTEIN GI NUMBER: 127138957
- NCBI GENE ID NUMBER: 57616
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- TSHZ3 Antibody: The Teashirt zinc finger homeobox (TSHZ) family comprise a family of evolutionarily conserved transcription factors that, in Drosophila, are active in specific body parts for patterning, but whose function in vertebrates is less clear. In mice, the known three TSHZ proteins are expressed in distinct patterns in the developing and adult brain, suggesting that they play a role in the establishment of regional identity and specification of cell types within the brain. Both TSHZ3 and TSHZ1 have been found to interact with FE65, an adapter protein that binds to the amyloid protein precursor (APP) in neurons. Together with SET, a component of the inhibitor of acetyl transferase, and histone deacetylases, these proteins formed a gene-silencing complex whose target includes caspase-4. Recent experiments have also suggested this family of proteins may be involved in carcinogenesis.
- 1: Caubit X, Core N, Boned A, et al. Vertebrate orthologues of the Drosophila region-specific patterning gene teashirt. Mech. Dev. 2000; 91:445-8.
- 2: Santos JS, Fonseca NA, Vieira CP, et al. Phylogeny of the Teashirt-related zinc finger (tshz) gene family and analysis of the developmental expression of tshz2 and tshz3 in the zebrafish. Dev. Dyn. 2010; 239:1010-8.
- 3: Caubit X, Tiveron MC, Cremer H, et al. Expression patterns of the three Teashirt-related genes define specific boundaries in the developing and postnatal mouse forebrain. J. Comp. Neurol. 2005; 486:76-88.
- 4: Kajiwara Y, Akram A, Katsel P, et al. FE65 binds teashirt, inhibiting expression of the primate-specific caspase-4. PLoS One 2009; 4:e5071
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 6759
- Size: 0.02 mg | 0.1 mg
- List Price: $109.00 – $379.00
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