- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: Rabbit polyclonal TSC22D3 antibody was raised against a 16 amino acid peptide near the amino terminus of human TSC22D3.
The immunogen is located within amino acids 40 - 90 of TSC22D3.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: TSC22D3 antibody can be used for detection of TSC22D3 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- POSITIVE CONTROL 1: Cat. No. 1308 - Human Small Intestine Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 22 kDa
Observed: 23 kDa
- PURIFICATION: TSC22D3 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TSC22D3 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TSC22D3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
- NCBI OFFICIAL SYMBOL: TSC22D3
- ADDITIONAL NAMES: TSC22D3 Antibody: DIP, GILZ, hDIP, DSIPI, TSC-22R, TSC22 domain family protein 3, DSIP-immunoreactive peptide, Protein DIP
- Protein Accession Number: NP_932174
- PROTEIN GI NUMBER: 37622903
- NCBI GENE ID NUMBER: 1831
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- TSC22D3 Antibody: The TSC22 domain family member 3 protein (TSC22D3) is a leucine zipper protein that functions as a transcriptional regulator. The expression of TSC22D3 is stimulated by glucocorticoids and IL-10 and is thought to play a key role in the anti-inflammatory and immunosuppressive effects of these molecules. TSC22D3 can physically interact with and inhibit the activities of key inflammatory signaling mediators such NF-κB and AP-1. TSC22D3 functions as a transcriptional co-activator for various nuclear receptors and NF-κB. It has also been shown to be involved in the differentiation of mesenchymal stem cells towards osteoblasts and bone formation.
- 1: Riccardi C, Cifone MG, and Migliorati G. Glucocorticoid hormone-induced modulation of gene expression and regulation of T-cell death: role of GITR and GILZ, two dexamethasone-induced genes. Cell Death Differ. 1999; 6:1182-9.
- 2: Berrebi D, Bruscoli S, Cohen N, et al. Synthesis of glucocorticoid-induced leucine zipper (GILZ) by macrophages: an anti-inflammatory and immunosuppressive mechanism shared by glucocorticoids and IL-10. Blood 2003; 101:729-38
- 3: Ayroldi E, Migliorati G, Bruscoli S, et al. Modulation of T-cell activation by the glucocorticoid-induced leucine zipper factor via inhibition of nuclear factor kappaB. Blood 2001; 98:743-53.
- 4: Mittelstadt PR and Ashwell JD. Inhibition of AP-1 by the glucocorticoid-inducible protein GILZ. J. Biol. Chem. 2001; 276:29603-10.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 7221
- Size: 0.02 mg | 0.1 mg
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