Tryptophan (phospho Ser58) Hydroxylase Antibody
CATALOG NUMBER: XPS-1030- Clonality: Polyclonal
- Tested Applications: WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.1 mL
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: TPH (Ser58) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphoSer58 of TPH.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: Applications include Dot Blots (DB) and Western Blots (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
- SPECIFICITY: Tryptophan Hydroxylase antibody is specific immunolabeling of the ~55k TPH protein phosphorylated at Ser58 in Western blots of human dorsal Raphe nucleus.
- PREDICTED MOLECULAR WEIGHT: 55
Properties
- PURIFICATION: Affinity Purified
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Additional Info
- NCBI OFFICIAL SYMBOL: Tph1
- Protein Accession Number: P09810
- PROTEIN GI NUMBER: 136122
- NCBI GENE ID NUMBER: 24848
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH.
- 1: Martinez, A., Knappskog, P.M. and Haavik, J. "A structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis," Curr. Med. Chem., 8 (2001) 1077 - 1091.
- 2: Jiang, G.C., Yohrling, G.J., Schmitt, J.D. and Vrana, K.E., "Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling," J. Mol. Biol., 302 (2000) 1005 - 1017.
- 3: Banik, U., Wang, G.A., Wagner, P.D., and Kaufman, S., "Interaction of phosphorylated tryptophan hydroxylase with 14-3-3 proteins," J. Biol. Chem. 272 (1997) 26219-26225.
- 4: Kuhn, D.M., Arthur, Jr., R., and States, J.C., "Phosphorylation and activation of brain tryptophan hydroxylase: identification of serine-58 as a substrate site for protein kinase A," J. Neurochem. 68 (1997) 2220 - 2223.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
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