- Trial Size
TRPC3 Antibody
CATALOG NUMBER: 3905- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (100%)
- IMMUNOGEN: TRPC3 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TRPC3.
The immunogen is located within amino acids 760 - 810 of TRPC3. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1303 - Human Brain Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1401 - Mouse Heart Tissue Lysate
Properties
- PURIFICATION: TRPC3 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TRPC3 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TRPC3 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: TRPC3
- ADDITIONAL NAMES: TRPC3 Antibody: TRP3, TRP3, Short transient receptor potential channel 3, Transient receptor protein 3, TrpC3
- Protein Accession Number: CAA74083
- PROTEIN GI NUMBER: 2225937
- NCBI GENE ID NUMBER: 7222
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- TRPC3 Antibody: The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.
- 1: Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31.
- 2: Trebak M, Vazquez G, Bird GSJ, et al. The TRPC3/6/7 subfamily of cation channels. Cell Calcium 2003; 33:451-61.
- 3: Zitt C, Obukhov AG, Strubing C, et al. Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion. J. Cell. Biol. 1997; 1333-41.
- 4: Dietrich A, Kalwa H, Rost BR, et al. The diacylglycerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance. Pflugers Arch. 2005; 451:72-80.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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