TriMethyl-Histone H3-K4 pAb
CATALOG NUMBER: 18-620- Clonality: Polyclonal
- Tested Applications: ChIP, ChIPseq, IF, IHC, IP, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Other, Rat
- Conjugate: Unconjugated
- Size: 50 uL
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Other, Rat
- IMMUNOGEN: A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ChIP, ChIPseq, IF, IHC, IP, WB
- APPLICATION NOTE: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
ChIP: 1:20 - 1:100
CHIP seq: 1:20 - 1:100 - POSITIVE CONTROL 1: HeLa
- POSITIVE CONTROL 2: H3 protein
- PREDICTED MOLECULAR WEIGHT: Observed: 18kDa
Properties
- PURIFICATION: Affinity purification
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at -20°C. Avoid freeze / thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: H3K4me3
- ADDITIONAL NAMES: H3F3A, H3t, H3.4, H3/g, H3FT
- NCBI GENE ID NUMBER: 8290
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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