- Trial Size
TRIM5 alpha Antibody
CATALOG NUMBER: 3251- Clonality: Polyclonal
- Tested Applications: E, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: TRIM5 alpha antibody was raised against a synthetic peptide corresponding to 19 amino acids near the center of rhesus monkey TRIM5 alpha.
The immunogen is located within amino acids 390 - 440 of TRIM5 alpha. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC-P, WB
- APPLICATION NOTE: TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. - SPECIFICITY: TRIM5 alpha antibody will not cross-react with other isoforms of TRIM5.
- POSITIVE CONTROL 1: Cat. No. 1319 - Human Stomach Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1317 - Human Uterus Tissue Lysate
- POSITIVE CONTROL 3: Cat. No. 1314 - Human Thymus Tissue Lysate
- POSITIVE CONTROL 4: Cat. No. 11-401 - Human Uterus Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 54 kDa
Observed: 54 kDa
Properties
- PURIFICATION: TRIM5 alpha Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TRIM5 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TRIM5 alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: TRIM5
- ADDITIONAL NAMES: TRIM5 alpha Antibody: RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88
- Protein Accession Number: ABG67967
- PROTEIN GI NUMBER: 110339457
- NCBI GENE ID NUMBER: 574288
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.
- 1: Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.
- 2: Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5a restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
- 3: Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5α. Proc. Nat'l. Acad. Sci. USA 2004; 101:10774-9
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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