- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: TRIM24 antibody was raised against a 19 amino acid peptide near the center of human TRIM24.
The immunogen is located within amino acids 530 - 580 of TRIM24.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, ICC, IF, WB
- APPLICATION NOTE: TRIM24 antibody can be used for detection of TRIM24 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
- SPECIFICITY: At least two isoforms of TRIM24 are known to exist.
- POSITIVE CONTROL 1: Cat. No. 1287 - EL4 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-207 - EL4 Cell Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 112, 116
Observed: 108, 1 of 2 isoforms
- PURIFICATION: TRIM24 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TRIM24 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TRIM24 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
- NCBI OFFICIAL SYMBOL: TRIM24
- ADDITIONAL NAMES: TRIM24 Antibody: PTC6, TF1A, TIF1, RNF82, TIF1A, hTIF1, TIF1ALPHA, Transcription intermediary factor 1-alpha, E3 ubiquitin-protein ligase TRIM24, TIF1-alpha
- Protein Accession Number: NP_056989
- PROTEIN GI NUMBER: 47419911
- NCBI GENE ID NUMBER: 8805
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- TRIM24 Antibody: TRIM24 (TIF1), a member of the tripartite motif (TRIM) family, plays a role in the regulation of cell proliferation and apoptosis. TRIM24 localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The TRIM motif includes three zinc-binding domains (RING, B-box type 1 and B-box type 2) and a coiled-coil region. TIF1 mediates transcriptional events by interactions with the AF2 region of several nuclear receptors, such as the estrogen, retinoic acid and vitamin D3 receptors. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC).
- 1: Fraser RA, Heard DJ, Adam S, et al. The putative cofactor TIF1 is a protein kinase that is hyperphosphorylated upon interaction with liganded nuclear receptors. J. Biol. Chem. 1998; 273:16199-204.
- 2: Tisserand J, Khetchoumian K, Thibault C, et al. Tripartite motif 24 (Trim24/Tif1a) tumor suppressor protein is a novel negative regulator of interferon (IFN)/signal transducers and activators of transcription (STAT) signaling pathway acting through retinoic acid receptor a (Rara) inhibition. J. Biol. Chem. 2011; 286:33369-79.
- 3: Herquel B, Ouararhni K, Khetchoumian K, et al. Transcription cofactors TRIM24, TRIM28, and TRIM33 associate to form regulatory complexes that suppress murine hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 2011; 108:8212-7.
- 4: Klugbauer S and Rabes HM. The transcription co-activator HTIF1 and a related protein are fused to the RET receptor tyrosine kinase in childhood papillary thyroid carcinomas. Oncogene 1999;18:4388-93.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 7383
- Size: 0.02 mg | 0.1 mg
- List Price: $109.00 – $379.00
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