- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (76%)
- IMMUNOGEN: TOX antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TOX.
The immunogen is located within amino acids 130 - 180 of TOX.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, WB
- APPLICATION NOTE: TOX antibody can be used for detection of TOX by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- SPECIFICITY: No alternatively spliced transcript variants have been observed.
- POSITIVE CONTROL 1: Cat. No. 1320 - Human Colon Tissue Lysate
- PREDICTED MOLECULAR WEIGHT: 58 kDa
- PURIFICATION: TOX Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TOX Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TOX antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: TOX
- ADDITIONAL NAMES: TOX Antibody: TOX1, KIAA0808, Thymocyte selection-associated high mobility group box protein TOX, Thymus high mobility group box protein TOX
- Protein Accession Number: NP_055544
- PROTEIN GI NUMBER: 7662322
- NCBI GENE ID NUMBER: 9760
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- TOX Antibody: TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.
- 1: Wilkinson B, Chen JY, Han P, et al. TOX: an HMG box protein implicated in the regulation of thymocyte selection. Nat. Immunol. 2002; 3:272-80.
- 2: Saito T and Watanabe N. Positive and negative thymocyte selection. Crit. Rev. Immunol. 1998; 18:359-70
- 3: Aliahmad P, O'Flaherty E, Han P, et al. TOX provides a link between calcineurin activation and CD8 lineage commitment. J. Exp. Med. 2004; 199:1089-99.
- 4: Aliahmad P, Kadavallore A, de la Torre B, et al. TOX is required for development of the CD4 T cell lineage gene program. J. Immunol. 2011; 187:5931-40.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 6987
- Size: 0.02 mg | 0.1 mg
- List Price: $89.00 – $395.00
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