- Trial Size
TOX Antibody
CATALOG NUMBER: 6987- Clonality: Polyclonal
- Tested Applications: E, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (76%)
- IMMUNOGEN: TOX antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TOX.
The immunogen is located within amino acids 130 - 180 of TOX. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, WB
- APPLICATION NOTE: TOX antibody can be used for detection of TOX by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: No alternatively spliced transcript variants have been observed.
- POSITIVE CONTROL 1: Cat. No. 1320 - Human Colon Tissue Lysate
- PREDICTED MOLECULAR WEIGHT: 58 kDa
Properties
- PURIFICATION: TOX Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TOX Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TOX antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: TOX
- ADDITIONAL NAMES: TOX Antibody: TOX1, KIAA0808, Thymocyte selection-associated high mobility group box protein TOX, Thymus high mobility group box protein TOX
- Protein Accession Number: NP_055544
- PROTEIN GI NUMBER: 7662322
- NCBI GENE ID NUMBER: 9760
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- TOX Antibody: TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.
- 1: Wilkinson B, Chen JY, Han P, et al. TOX: an HMG box protein implicated in the regulation of thymocyte selection. Nat. Immunol. 2002; 3:272-80.
- 2: Saito T and Watanabe N. Positive and negative thymocyte selection. Crit. Rev. Immunol. 1998; 18:359-70
- 3: Aliahmad P, O'Flaherty E, Han P, et al. TOX provides a link between calcineurin activation and CD8 lineage commitment. J. Exp. Med. 2004; 199:1089-99.
- 4: Aliahmad P, Kadavallore A, de la Torre B, et al. TOX is required for development of the CD4 T cell lineage gene program. J. Immunol. 2011; 187:5931-40.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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