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TOM70 Antibody
TOM70 Antibody
TOM70 Antibody
TOM70 Antibody
TOM70 Antibody
TOM70 Antibody
TOM70 Antibody
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TOM70 Antibody

CATALOG NUMBER: 4959
  • Clonality: Polyclonal
  • Tested Applications: ELISA, IF, IHC-P, WB
  • Host Species: Rabbit
  • Species Reactivity: Human, Mouse, Rat
  • Conjugate: Unconjugated
  • Size: 0.02 mg | 0.1 mg

Specifications

  • HOST SPECIES: Rabbit
  • SPECIES REACTIVITY: Human, Mouse, Rat
  • IMMUNOGEN: Anti-TOM70 antibody (4959) was raised against a peptide corresponding to 15 amino acids near the center of human TOM70.
    The immunogen is located within amino acids 220-270 of TOM70.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
  • APPLICATION NOTE: WB: 1-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
    Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested.
  • POSITIVE CONTROL 1: Cat. No. 1303 - Human Brain Tissue Lysate
  • POSITIVE CONTROL 2: Cat. No. 1403 - Mouse Brain Tissue Lysate
  • POSITIVE CONTROL 3: Cat. No. 1463 - Rat Brain Tissue Lysate
  • POSITIVE CONTROL 4: Cat. No. 1202 - A431 lysate
  • POSITIVE CONTROL 5: Cat. No. 1224 - Daudi lysate
  • POSITIVE CONTROL 6: Cat. No. 1282 - 3T3/NIH lysate
  • PREDICTED MOLECULAR WEIGHT: Predicted: 68kD
    Observed: 72 kD

Advanced Validation

  • VALIDATION:

    Independent Antibody Validation in Cell lines (Figure 1) shows similar TOM70 expression profile in human, mouse and rat cell lines detected by two independent anti-TOM70 antibodies that recognize different epitopes, 4949 against N-terminus domain and 4959 against central domain.TOM70 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

  • ISOFORMS: Human TOM70 has only one isoform (608aa, 67.5kD. Mouse TOM70 has one isoform (611aa, 67.6kD) and Rat TOM70 also has one isoform (610aa, 67.4kD). 4959 can detect human, mouse and rat.

Properties

  • PURIFICATION: TOM70 Antibody is affinity chromatography purified via peptide column.
  • CLONALITY: Polyclonal
  • ISOTYPE: IgG
  • PHYSICAL STATE: Liquid
  • BUFFER: TOM70 Antibody is supplied in PBS containing 0.02% sodium azide.
  • CONCENTRATION: 1 mg/mL
  • STORAGE CONDITIONS: TOM70 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

  • NCBI OFFICIAL SYMBOL: TOMM70A
  • ADDITIONAL NAMES: TOM70 Antibody: KIAA0719, TOM70, Mitochondrial import receptor subunit TOM70, Mitochondrial precursor proteins import receptor
  • Protein Accession Number: NP_055635
  • PROTEIN GI NUMBER: 54607135
  • NCBI GENE ID NUMBER: 9868
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • TOM70 Antibody: The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM70, an important member of the TOM complex, contains a tetratricopeptide repeat domain similar to those found in cytosolic chaperones such as Hsp90 and Hsc70 and provides a docking site for these proteins. This interaction is thought to be a critical first step in the TOM70-dependent mitochondrial import, followed by contact between the preprotein and TOM70. After targeting to TOM70, preproteins are translocated through the outer membrane via the TOM40 import pore complex. The precise mechanism of how preproteins progress from TOM70 to TOM40 to full translocation is still unclear. At least two isoforms of TOM70 are known to exist.
  • 1: Kutik et al. J. Cell Biol. 2007; 179:585-91.
  • 2: Young et al. Cell 2003; 112:41-50.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 4959

  • Size: 0.02 mg | 0.1 mg
  • List Price: $89.00 – $395.00
Datasheet
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