TNF-a Antibody (biotin)
CATALOG NUMBER: XP-5283Bt- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Biotin
- Size: 0.05 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTNF-alpha (human hTNF-alpha).
- CONJUGATE: Biotin
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: ELISA:
Sandwich:
To detect hTNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TNF-α (XP-5283) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-α.
Western Blot:
To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: Anti-hTNF-alpha specific antibody was purified by affinity chromatography and then biotinylated.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: TNF-a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: TNF
- ADDITIONAL NAMES: DIF, TNFA, TNFSF2, TNF-alpha, Tumor necrosis factor, Cachectin, TNF-a
- Protein Accession Number: P01375
- PROTEIN GI NUMBER: 135934
- NCBI GENE ID NUMBER: 7124
- USER NOTE: Centrifuge vial prior to opening.
Background
- Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.
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