- Trial Size
TLR2 Antibody
CATALOG NUMBER: 3135- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: TLR2 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TLR2.
The immunogen is located within the first 50 amino acids of TLR2. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: TLR2 antibody can be used for detection of TLR2 by Western blot at 1 μg/mL. Despite its predicted molecular weight, TLR2 runs at ~100 kDa in SDS-PAGE. This polyclonal antibody can also detect TLR2 by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1288 - A20 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1306 - Human Spleen Tissue Lysate
- POSITIVE CONTROL 3: Cat. No. 10-901 - Human Spleen Tissue Slide
Properties
- PURIFICATION: TLR2 Antibody is Ion exchange chromatography purified.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TLR2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TLR2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: TLR2
- ADDITIONAL NAMES: TLR2 Antibody: TIL4, CD282, TIL4, Toll-like receptor 2
- Protein Accession Number: O60603
- PROTEIN GI NUMBER: 20140434
- NCBI GENE ID NUMBER: 7097
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- TLR2 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-κB. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer can recognize a variety of bacterial and mycoplasma lipoproteins respectively.
- 1: Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
- 2: Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
- 3: Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
- 4: O'Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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