- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: TIRAP antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of mouse TIRAP.
The immunogen is located within the last 50 amino acids of TIRAP.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: TIRAP antibody can be used for detection of TIRAP by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- SPECIFICITY: At least three isoforms of TIRAP are know to exist; this antibody detects all three isoforms.
- POSITIVE CONTROL 1: Cat. No. 1301 - Human Heart Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-501 - Human Heart Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 24, 26 kDa
Observed: 24 kDa
- PURIFICATION: TIRAP Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TIRAP Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TIRAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: TIRAP
- ADDITIONAL NAMES: TIRAP Antibody: Mal, wyatt, BACTS1, MyD88-2, MAL, Adaptor protein Wyatt, TIR domain-containing adapter protein
- Protein Accession Number: NP_473437
- PROTEIN GI NUMBER: 15705407
- NCBI GENE ID NUMBER: 114609
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- TIRAP Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors. In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling. Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-kappaB and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling.
- 1: Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
- 2: Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
- 3: Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
- 4: O'Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 3157
- Size: 0.02 mg | 0.1 mg
- List Price: $89.00 – $395.00
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