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TIM3 Antibody [9G1]
TIM3 Antibody [9G1]
TIM3 Antibody [9G1]
TIM3 Antibody [9G1]
TIM3 Antibody [9G1]
  • Trial Size

TIM3 Antibody [9G1]

CATALOG NUMBER: RF16109
  • Clonality: Monoclonal
  • Tested Applications: E, Flow, ICC, IF, IHC-P
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 0.02 mg | 0.1 mg

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: TIM-3 antibody was raised against the extracellular domain of human TIM-3
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: E, Flow, ICC, IF, IHC-P
  • APPLICATION NOTE: TIM-3 antibody can be used for ELISA starting at 0.1 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL. For Flow Cytometry start at 0.1 μg/ml.

Properties

  • PURIFICATION: TIM-3 Antibody is supplied as protein A purified IgG2b.
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG2b,k
  • PHYSICAL STATE: Liquid
  • BUFFER: TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
  • CONCENTRATION: 1 mg/mL
  • STORAGE CONDITIONS: TIM-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

  • NCBI OFFICIAL SYMBOL: HAVCR2
  • ADDITIONAL NAMES: TIM-3 Antibody: Hepatitis A virus cellular receptor, HAVCR2, TIM3, CD366, KIM-3, TIMD3, TIMD-3
  • Protein Accession Number: NP_116171
  • PROTEIN GI NUMBER: 84868
  • NCBI GENE ID NUMBER: 84868
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • The immune checkpoint protein TIM3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).
  • 1: Monney L, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of autoimmune disease. Nature 2002; 415:536-41.
  • 2: Sakuishi K, Apetoh L, Sullivan JM, et al. Targeting Tim-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tumor immunity. J Exp Med 2010; 207:2187–94.
  • 3: Zhou Q, Munger ME, Veenstra RG, et al. Coexpression of Tim-3 and PD-1 identifies a CD8+ T-cell exhaustion phenotype in mice with disseminated acute myelogenous leukemia. Blood 2011; 117:4501–10.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: RF16109

  • Size: 0.02 mg | 0.1 mg
  • List Price: $129.00 – $439.00
Datasheet
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